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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Mol Oncol. 2008 Aug;2(2):164–181. doi: 10.1016/j.molonc.2008.05.001

Figure 8.

Figure 8

Knock-down of PML decreases SnoN-induced increases in p21 levels. A-B, Both control and SnoN-EGFP F-11 cells were transfected with PML siRNA. Following 24 and 48 hours transfection with PML siRNA, RNA and protein were harvested, respectively. qPCR was performed using PML qPCR probes/primers to measure RNA-fold changes in PML RNA levels and western blotting was performed using antibodies against PML, SnoN, p21, and GAPDH as a loading control. C, Control and PML siRNA treated TIOSE cells at 24 hours post-transfection, were re-seeded on to 96 well plates at 5000 cells were well and allowed to grow for another 48 hours. Growth rate was measured by MTS assay.