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. Author manuscript; available in PMC: 2014 Dec 23.
Published in final edited form as: Mol Ther Methods Clin Dev. 2014 Oct 15;3:14049. doi: 10.1038/mtm.2014.49

Figure 2. Surgery and vector-related histological findings.

Figure 2

Independent evaluation of hematoxylin and eosin (H&E) staining of coronal sections containing the cannula tract revealed normal gliosis related to cannula insertion in all experimental groups (arrowheads). Higher magnification images (right column) were taken close to the cannula tip (black arrows in left column). H&E staining also showed perivascular cellular infiltrates in AAV2-hAADC-treated, but not PBS-treated, animals regardless of survival time. Although incidence and severity of perivascular cuffs was increased with AAV2-hAADC vector dose, these were not considered adverse. Note that there were also many vessels with no perivascular cuffing close to the infusion site (white arrows in left column) and that perivascular cellular infiltrates were not present in the pilot AAV2-hAADC animal. Scale bars: left column: 1 cm; right column: 100 μm.