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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: MethodsX. 2014;1:269–274. doi: 10.1016/j.mex.2014.10.007

Fig. 3.

Fig. 3

Aβ immunostaining of free floating sections following different antigen retrieval methods. Wild type (WT) and APP/PS1 free floating 40 μm tissue sections were untreated (no treatment) or processed through single or combined antigen retrieval methods (trypsin-EDTA, 10 mM EDTA pH 6.0, formic acid, combined treatment) before immunostaining using anti-Aβ antibody. Representative Aβimmunostained images visualized using vector VIP as the chromogen taken from the temporal cortex are shown from (a) wild type (WT) no treatment control, (b) APP/PS1 no treatment control, (c) WT trypsin-EDTA antigen retrieval, (d) APP/PS1 trypsin-EDTA antigen retrieval, (e) WT 10 mM EDTA, pH 6.0 antigen retrieval, (f) APP/PS1 10 mM EDTA, pH 6.0 antigen retrieval, (g) WT formic acid antigen retrieval, (h) APP/PS1 formic acid antigen retrieval, (i) WT combined treatment antigen retrieval, and (j) APP/PS1 combined treatment antigen retrieval.