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. Author manuscript; available in PMC: 2015 Oct 17.
Published in final edited form as: J Am Chem Soc. 2014 Nov 19;136(48):16832–16843. doi: 10.1021/ja5076184

Figure 3.

Figure 3

Freely diffusing smFRET experiments reveal the Mg2+-dependent formation of the docked conformation in the a) absence and b) presence of 1000 nM HyCbl. Note the color scheme in a–b) is only intended to represent the increasing [HyCbl]; colors do not necessarily correlate one-to-one with [HyCbl]. c) The presence of saturating HyCbl (1000 nM, bronze circles) decreases the concentration of Mg2+ required to achieve half-maximal occupancy of the docked conformation (i.e., [Mg2+]½) by ≈ 10-fold. The vertical dashed bar represents near-physiological salt conditions (see text for details). The error bars are smaller than the symbols used for each data point (Materials and Methods).