Figure 3.
31P NMR spectra of the products obtained by incubation of His6-FzmG with Me-PnA. A. NMR spectrum of the reaction mixture containing Me-PnA, O2, α-KG, Fe(II), L-ascorbate, and His6-FzmG. B. NMR spectrum of the enzymatic reaction mixture spiked with authentic standard of Me-HPnA. C. NMR spectrum of the reaction mixture in the absence of α-KG. D. NMR spectrum of the reaction mixture in the absence of L-ascorbate. E. NMR spectrum of the reaction mixture in the absence of His6-FzmG. F. NMR spectrum of authentic Me-HPnA.