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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Cell Mol Gastroenterol Hepatol. 2015 Jan;1(1):55–74.e1. doi: 10.1016/j.jcmgh.2014.08.001

Figure 5. Intracolonic cathelicidin peptide administration reduces colonic collagen deposition in cathelicidin deficient mice with chronic TNBS colitis.

Figure 5

(A) Representative H&E images of colonic tissues. (B) Histology score was based on H&E staining images of colonic tissues. TNBS treatment in Camp−/− mice led to increased tissue damage with significantly higher histology score (p=0.001), compared to ethanol control. Peptide treatment of mCRAMP significantly reduced histology score in Camp−/− mice (p=0.04). (C) Masson Trichrome staining for collagen in colonic tissues. Collagen was stained in blue. Collagen deposited in mucosal and submucosal layer of TNBS treated Camp−/− mice was reduced by mCRAMP peptide treatment. (D) Quantitative real-time RT-PCR of collagen col1a2 mRNA expression in colonic tissues of mice. TNBS treatment significantly induced colonic col1a2 mRNA (p=0.0311) expression. Intracolonic treatment of mCRAMP reduced colonic col1a2 mRNA expression in Camp−/− (p=0.04) mice. (E) TNBS treatment significantly induced colonic Tnfα protein (p=0.0042 in WT and p=0.0241 in Camp−/− mice) expression. Intracolonic mCRAMP administration significantly reduced TNBS induced Tnfα protein expression in Camp−/− (p=0.0054) mice. n=6 mice per group.