Figure 4. Syk deficiency hinders robust activation of SM1T cells in vivo.

A) 1×10⁶ CFSE stained SM1 T cells were adoptively transferred into wild-type and Syk-deficient chimeric mice and following day were immunized with 1μg of flagellin. Three days later clonal expansion of SM1 T cells was examined in the spleen. B–C) Bar diagrams represent the percentage and total SM1 T cells in the spleen of wild-type or Syk –deficient mice immunized with flagellin (1μg). P<0.05 (**). (D–E) SM1 T cell proliferation in wild-type (solid line) and Syk deficient (dotted line) mice was examined by CFSE dye-dilution. Each plot is a representative of three mice per group and three independent experiments.