. Author manuscript; available in PMC: 2009 Aug 15.

Published in final edited form as: Virology. 2008 Jun 25;378(1):105–117. doi: 10.1016/j.virol.2008.04.043

Table III.

Results of class II binding assay for the 15mer peptides from the yellow fever capsid protein

Position⁽¹⁾	Sequence	Binding Assay (IC₅₀ in nmol)
Position⁽¹⁾	Sequence	I-A^d⁽²⁾	I-E^d
C_1–15	SGRKAQGKTLGVNMV	3810⁽³⁾	>45000
C_5–19	AQGKTLGVNMVRRGV	1547	16452
C_9–23	TLGVNMVRRGVRSLS	56^*	9379
C_13–27	NMVRRGVRSLSNKIK	801^*	11260
C_17–31	RGVRSLSNKIKQKTK	38215	5697
C_21–35	SLSNKIKQKTKQIGN	>45000	18106
C_25–39	KIKQKTKQIGNRPGP	25182	24418
C_29–43	KTKQIGNRPGPSRGV	323^*	33245
C_33–47	IGNRPGPSRGVQGFI	9683	>45000
C_37–51	PGPSRGVQGFIFFFL	11783	>45000
C_41–55	RGVQGFIFFFLFNIL	9301	>45000
C_45–59	GFIFFFLFNILTGKK	3535	>45000
C_49–63	FFLFNILTGKKITAH	562^*	992^*
C_53–67	NILTGKKITAHLKRL	378^*	8234
C_57–71	GKKITAHLKRLWKML	5867	389^*
C_61–75	TAHLKRLWKMLDPRQ	>45000	3788
C_65–79	KRLWKMLDPRQGLAV	539^*	6193
C_69–83	KMLDPRQGLAVLRKV	2523	>45000
C_73–87	PRQGLAVLRKVKRVV	22243	1844
C_77–91	LAVLRKVKRVVASLM	93^*	1081
C_81–95	RKVKRVVASLMRGLS	60^*	6687
C_85–99	RVVASLMRGLSSRKR	41^*	522^*
C_89–103	SLMRGLSSRKRRSHD	1326	545^*
C_93–107	GLSSRKRRSHDVLTV	17646	13392
C_97–111	RKRRSHDVLTVQFLI	1631	14460
C_101–115	SHDVLTVQFLILGML	29907	>45000
C_105–119	LTVQFLILGMLLMTG	37394	>45000
C_109–120	FLILGMLLMTGG	>45000	>45000

⁽¹⁾

Position of each 15mer peptide in the capsid protein (C) of the yellow fever virus

⁽²⁾

molecules of H2^d class II

⁽³⁾

IC₅₀ result for the binding assay for H2^d class II molecules (nmol);

values of IC₅₀ below the cutoff of 1000nmol, suggestive of a possible binder peptide; Shading – peptides positive for CD4 response by ELISPOT (Fig. 6A)