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. Author manuscript; available in PMC: 2015 Oct 5.
Published in final edited form as: Cancer Sci. 2015 Feb 12;106(3):227–236. doi: 10.1111/cas.12593

Figure 4. Functional domains of PU.1.

Figure 4

(A) Deletion mutants of PU.1. The MOZ- and MLL-interacting domains and the results for interaction with PU.1 and PU.1-mediated transactivation of Csf1r-luc are indicated. ND, not determined (B) Interaction of PU.1 mutants with MLL. 293T cells were co-transfected with HA-tagged MLL and wild-type or mutants of FLAG-tagged PU.1. Anti-FLAG antibody immunoprecipitates (IP:FLAG) or cell lysates (Input) were subjected to immunoblotting with anti-HA or anti-FLAG antibodies. (C) SaOS2 cells were co-transfected with the Csf1r–luciferase construct and the indicated effectors. Luciferase activity was analyzed 24 h after transfection. Error bars represent SD (n = 3).