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. Author manuscript; available in PMC: 2009 Feb 13.
Published in final edited form as: J Neurosci. 2008 Aug 13;28(33):8306–8315. doi: 10.1523/JNEUROSCI.2614-08.2008

Figure 3. Comparison of Fm/Fc between anterogradely-moving, retrogradely-moving, and stationary mitochondria shows no difference in Fm/Fc.

Figure 3

Mitochondria were stained with both Mitotracker and TMRM. (A, B) Imaging of Mitotracker shows the position mitochondria along the axon; these two images were acquired 60 seconds apart. A single moving mitochondrion is highlighted with an arrow. (C) TMRM staining of the same field in A indicates Fm/Fc. Scale bar =10 μm. (D) Comparisons of populations of mitochondria using a paired t-test shows that the Fm/Fc does not vary between stationary and moving mitochondria, or between anterogradely- and retrogradely-moving mitochondria. n = 33 and 21 cells for that contained anterogradely- or retrogradely-moving mitochondria respectively.