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. Author manuscript; available in PMC: 2015 Nov 14.
Published in final edited form as: Bioconjug Chem. 2014 Nov 25;25(12):2116–2122. doi: 10.1021/bc5004923

Figure 2. Induction of chemotaxis and ROS production of murine neutrophils by fMLF-derived hydrogelators (1, 2 and 3) in vitro.

Figure 2

(A) Snapshots of chemotaxis of murine neutrophils at 0, 5 and 10 minutes induced by the gradient of fMLF, 1, 2 and 3 performed on EZ-TAXIScan,(40) with the blank control. (B) The traces of 20 typical migrating neutrophils corresponding to different chemoattractants in the first 20 minutes. (C) The scheme and formulas for the calculation of the migration parameters. (D) The migration speed, (E) directionality, and (F) upward directionality of the murine neutrophils during chemotaxis from three independent samples, and each sample has 20 cell traces. (G) ROS production in the neutrophils (5 × 105) after stimulation with DMSO (0.1 v/v%, as the negative control), fMLF (10 µM), 1 (1 µM), 2 (10 µM), and 3 (10 µM).