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. Author manuscript; available in PMC: 2015 Nov 14.
Published in final edited form as: Bioconjug Chem. 2014 Nov 25;25(12):2116–2122. doi: 10.1021/bc5004923

Figure 3. Induction of chemotaxis and ROS production of human neutrophils by 3 in vitro.

Figure 3

(A) Snapshots of chemotaxis of purified neutrophils from healthy human donors at 0, 5 and 10 minutes induced by fMLF, 3, and PBS (as the control) performed on EZ-TAXIScan. (B) The traces of 20 typical migrating neutrophils corresponding to different chemoattractants. (C) The migration speed, (D) directionality and (E) upward directionality of the human neutrophils during chemotaxis from three independent samples, and each sample has 20 cell migration traces. (F) ROS production in the neutrophils (5 × 105) after the addition of fMLF (100 nM), 3 (100 nM), and PBS (as the blank control).