. Author manuscript; available in PMC: 2015 May 6.

Published in final edited form as: J Vis Exp. 2015 Apr 14;(98):10.3791/52503. doi: 10.3791/52503

Table 2.

Quantitative measures of deuterium uptake in ssHDX studies of Mb formulations.

	Deuterium Uptake (%)^b	N_fast^c	k_fast^c	N_slow^c	k_slow^c
MbT^a	15.9 ± 0.5	13.1 (0.8)	0.43 (0.03)	11.0 (0.9)	0.019 (0.001)
MbS^a	23.2 ± 0.5	15.4 (0.7)	0.49 (0.04)	19.2 (0.6)	0.024 (0.002)
% change^d	46%	18%	14%	75%	26%

See Table 1 for composition.

Percent deuterium uptake relative to theoretical maximum by intact Mb after 144 h of HDX at 5 °C, 43% RH (n = 3, mean ± SD).

Parameters determined by nonlinear regression of ssHDX-MS kinetic data. Time course of deuterium exchange for intact Mb was fitted to a biexponential association model (Eqn. 2). Values in parentheses are standard errors of the regression parameters.

The percent change in measurements were calculated as 100 × [(value from MbS – value from MbT)/ (value from MbT)].