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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Chem Sci. 2015 Mar;6(3):1979–1985. doi: 10.1039/c4sc03516j

Figure 5.

Figure 5

Imaging H2S using CHS-3. (a) Images 30 sec after adding 40 μM CHS-3 to 0, 25, 50, 100, and 200 μM Na2S in 20 mM HEPES buffer (pH 7.4) containing 20% Emerald II Enhancer (n = 3). (b) Plot of total photon flux versus H2S concentration for the experiments described in (a), (c)–(d) Images of living C6 brown mice 30 sec after administering i.p. injections of 0.08 μmol CHS-3 (and (c) vehicle control or (d) 0.4 μmol Na2S in 100 uL 20 mM HEPES buffer (pH 7.4) containing 20% Emerald II Enhancer, (e) Quantification of the total photon flux from three replicates of the experiments described in (c) and (d). Statistical analyses were performed with a two-tailed Student's t-test (n = 3, p = 0.025). Error bars are ±S.D.