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. Author manuscript; available in PMC: 2015 Oct 5.
Published in final edited form as: Cancer Sci. 2015 Mar 3;106(4):421–429. doi: 10.1111/cas.12610

Figure 2. Myc-5 binding at the target gene promoters.

Figure 2

A, EMSA of eIF4G1 gene match and mismatch promoter and with Myc-5 and mismatched PI polyamide. B, EMSA of eIF4G1 gene promoter with Myc-5 and mismatched PI polyamide. FITC-labeled hairpin oligonucleotide was incubated at 37°C for 60 min in Myc-5 or mismatched PI polyamide. C, Typical SPR sensograms for the interaction of PI polyamides with the hairpin duplex with 5’-biotin labeled and immobilized E-box (CACGTG) sequences. D–E, Remarkable differences in binding kinetics are observed: fast on/off kinetics for Myc-5 (D) and slower kinetics for the mismatch PI polyamide (E).