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. Author manuscript; available in PMC: 2015 Mar 25.
Published in final edited form as: J Immunol. 2015 Feb 20;194(7):3236–3245. doi: 10.4049/jimmunol.1402764

Figure 5. cGAS and Ifi204 cooperate to sense cytosolic dsDNA.

Figure 5

(A) Type I IFN levels were measured from wild-type RAW264.7 macrophages transfected for 24 h with U112 lysates either un-treated (UT) or treated with DNase I, heat-killed (HK) DNase I or RNase A. Type I IFN levels were measured from RAW264.7 macrophages transfected for 24 h with (B) endo-free plasmid DNA (pCherry), (C) 1 µg/mL Poly(I:C) or (D) 10 µg/mL c-di-GMP NT: not transfected. *p < 0.05, **p < 0.01, ***p < 0.001. Graphs show the mean ± standard deviation (SD) of triplicate wells and are representative of three independent experiments.