Fig. 7.

ETV1 overexpression alters genome-wide expression in Pten-hemizygous mice a Principal component analysis was performed for all genes among the individual RNA-seq libraries. hAR^12Q, hAR^21Q and hAR^48Q mice are represented as triangles, squares and circles, respectively. Green and red represent intact non-transgenic and transgenic mice, yellow and blue represent castrated non-transgenic and transgenic mice, and orange represents macroscopic tumors, respectively. b RNA-seq analysis was performed on DLP RNA as before. Heatmaps include differentially expressed genes between the groups shown (FDR ≤ 0.05), with columns representing the average among biological replicate libraries. By GLM analysis of all hAR;ETV1^Tg;Pten^+/− libraries vs. all hAR;Pten^+/− libraries, 59 genes are upregulated and 3 downregulated in prostates of hAR;ETV1^Tg;Pten^+/− mice. Select significantly enriched GO terms are listed. c ETV1-upregulated genes shared among Pten^+/− and Pten^+/+ mice are listed. d Differential expression was performed between transgenics and non-transgenics for each AR Q-tract group individually. Overlap between genes differentially expressed in hAR^12Q;ETV1^Tg, hAR^21Q;ETV1^Tg or hAR^48Q;ETV1^Tg relative to non-transgenics is illustrated by Venn diagram.