Table 1.

RNA from three separate sets of CGN cultures treated with HK or LK were used for RNA-Seq. Raw reads were mapped to the reference rat genome (rn4). Table 1.1 shows detailed mapping statistics of the sequencing reads obtained from the three sets of RNA samples in either condition. Differential expression analysis was performed using DEseq. Table 1.2 shows the numbers of genes differentially expressed after LK treatment of CGNs, calculated after applying FDR cutoff of adjusted p-value < 0.05

1.1
High Potasium (+25mM KCI, HK)
		Mapped			Uniquely mapped		% of Total mapped
	1	2	3	1	2	3	1	2	3
Exon-exon	4757117	4495316	3736405	4313864	4079542	3385667	16.91	16.87	16.01
Exon-intron	226170	199771	201238	215838	190137	192172	0.8	0.75	0.86
Total exon	23908062	22694951	19824076	22084046	20997447	18393002	84.96	85.19	84.97
Total intron	4231747	3944936	3507883	3460041	3206800	2897428	15.04	14.81	15.03
Total gene	28139809	26639887	23331959	25544087	24204247	21290430	100	100	100
Low Potasium (+5mM KCI, LK)
		Mapped			Uniquely mapped		% of Total mapped
	1	2	3	1	2	3	1	2	3
Exon-exon	4328289	4105310	3853533	3924946	3738989	3493850	16.27	16.11	15.29
Exon-intron	210037	199432	203481	199715	189776	193824	0.79	0.78	0.81
Total exon	22578563	21682543	21465130	20960096	20195084	20021671	84.89	85.08	85.2
Total intron	4018031	3802487	3729792	3184725	3000245	2983964	15.11	14.92	14.8
Total gene	26596594	25485030	25194922	24144821	23195329	23005635	100	100	100

1.2
Differential Expression	Gene Count
Up-regulated	2199
Down-regulated	2135
Total	4334