Skip to main content
. Author manuscript; available in PMC: 2015 Dec 29.
Published in final edited form as: Mol Cell. 2015 Feb 19;57(4):636–647. doi: 10.1016/j.molcel.2015.01.011

Figure 2. BRCA1 recruitment to the β–actin termination pause site is mediated by R-loops.

Figure 2

(A) RNA:DNA immunoprecipitation (DIP) analyses performed in HeLa from mock-treated or BRCA1-depleted cells (BRCA1 KD) (left) and mock-treated or SETX-depleted cells (SETX KD) (right).

(B) BRCA1 and SETX ChIP experiment performed in control conditions (-RNAseH1) or with RNAseH1 over-expression (+RNAseH1).

Average DIP and ChIP values ± SD from three, independent experiments are shown. p*<0.05.

(C) BRCA1 and SETX co-IP experiments performed in HeLa cells +/- RNAseH1 expression. IgG, negative control.