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. Author manuscript; available in PMC: 2015 Nov 18.
Published in final edited form as: ACS Chem Biol. 2014 Nov 18;10(2):510–516. doi: 10.1021/cb500683c

Table 1.

Michaelis–Menten Parameters of the Different Reactions Catalyzed by PHGDH, Compared to Intracellular Concentrations of the Corresponding Substrates and Products in Different Cell Lines

3-phosphoglycerate → 3-phosphohydroxypyravate oxaloacetate → malate α-ketoglutarate → 2-hydroxyglutarate
Kcat (1/min) 4.5 ± 0.6 Kcat (1/min) 10.6 ± 1.6 Kcat (1/min) 4.7 ± 0.9
Km, 3-phosphoglycerate (mM) 0.26 ± 0.034 Km, oxaloacetate (mM) 6.5 ± 1.3 Km, α-ketoglutarate (mM) 10.1 ± 1.8
[3-phosphoglycerate] (mM) MDA-MB-468 0.18 ± 0.01 [oxaloacetate] (mM) MDA-MB-468 not detectable [α-ketoglutarate] (mM) MDA-MB-468 0.55 ± 0.02
BT20 0.11 ± 0.01 BT20 not detectable BT20 0.47 ± 0.02
[3-phosphohydroxypyruvate] (mM) MDA-MB-468 not detectable [malate] (mM) MDA-MB-468 3.3 ± 0.08 [2-hydroxyglutarate] (mM) MDA-MB-468 0. 093 ± 0.009
BT20 not detectable BT20 0.99 ± 0.07 BT20 0.035 ± 0.002
Km, NAD+ (mM) 0.022 ± 0.003 Km, NADH (mM) 0.004 + 0.001 Km, NADH (mM) 0.004 ± 0.001
[NAD+] (mM) MDA-MB-468 0.99 ± 0.01 [NADH] (mM) MDA-MB-468 0.37 ± 0.02 [NADH] (mM) MDA-MB-468 0.37 ± 0.02
BT20 0.77 ± 0.02 BT20 0.186 ± 0.001 BT20 0.186 ± 0.001
[NADH] (mM) MDA-MB-468 0.37 ± 0.02 [NAD+] (mM) MDA-MB-468 0.99 ± 0.01 [NAD+] (mM) MDA-MB-468 0.99 ± 0.01
BT20 0.186 ± 0.001 BT20 0.77 ± 0.02 BT20 0.77 ± 0.02