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. Author manuscript; available in PMC: 2015 Jul 14.
Published in final edited form as: Biochemistry. 2014 Jul 18;53(30):5042–5050. doi: 10.1021/bi500545g

Figure 2.

Figure 2

2AA-GPP is an ineffective substrate for UPPSEc and UPPSVv. HPLC analysis of 2AA-GPP with (1, red) UPPSBf, (2, black) UPPSEc, and (3, blue) UPPSVv at (A) 167 nM and (B) 1.7 µM. Chromatograms 2 and 3 are offset by 2 and 4 min, respectively. Note that the peaks representing flow-through material in panel B (2AA-GPP) are cut to give the best view of long chain isoprenoid products. In all chromatograms, the ESI-MS-identified product is indicated below the time axis with the number of Z-configuration isoprenes associated with each peak.