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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Chem Sci. 2015 Feb 12;6(4):2457–2468. doi: 10.1039/c4sc03401e

Fig. 3.

Fig. 3

DNA damage rates and amounts of adducts from 2-AAF. Top panel - Relative DNA damage rate ({μg of protein}−1 s−1 mM−1) from ECL arrays exposed to 250 μM of 2-AAF in pH = 7.4 for (a) human organ tissue fractions, (b) cyt P450 supersomes. Bottom panel – Total amount of DNA adducts found (pmol {μg of protein}−1 {mM of 2-AAF}−1) after reactions with 250 μM 2-AAF at pH = 7.4 for 4 hrs, (c) human organ microsomes, (d) cyt P450 supersomes. The ECL images and single reaction monitoring LC-MS/MS chromatograms are in SI file (Figure S4 and Figure S7).