A, Four-year LMN denervated quadriceps muscle. H&E stain. Some of the severely atrophic myofibers have remnants of the contractile machinery, while others are empty of any sarcomeric structures.
B, Immunohistochemistry of the 4 years LMN denervated human muscle. Several atrophic slow type myofibers are stained in green by the anti-MHCslow antibody.
C, 4 years LMN denervated human muscle. A few muscle fibers are stained in green by the anti-MHCemb. Nuclei are stained by Hoechst 33258. Scale bar: 100 μm.