Fig. 4.

Immunofluorescence showing gross changes interaction between Golgi complex and ER distribution/morphology in response to KA-administration with PRNCs for 3 days at 37°C. PRNCs were fixed and stained for the Golgi complex and ER using antibodies against FTCD (green) and ERp29 (red), respectively, and for nuclei using Hoechst 33258 (blue). (A–C) Control, Golgi closely localized with ER which distributed around nuclei. (D–F) PRNCs were incubated with 5 µM KA for 3 days at 37°C. KA-induced epilepsy provoked Golgi complex fragmentation/dispersal, and ER are seen dislocated around nuclei. (G) Quantification of ER dislocation from nuclear surface corresponded to distance of reconstructed anti-ERp29 fluorescent signal from nuclear surface. **P < 0.01. Scale bars = 5µm.