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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Mol Oncol. 2015 Mar 31;9(7):1359–1370. doi: 10.1016/j.molonc.2015.03.009

Figure 4.

Figure 4

NF-κB inhibitor BAY11-7082 leads to selective decrease in proliferation and survival of VS cells. A. Representative proliferation images for primary VS cultures treated with (a) no treatment (NT), (b) 1 μM and (c) 5 μM BAY11-7082 (BAY11). BrdU in nuclei (red) marks proliferating cells, S100 (green) marks schwannoma cells. B. Quantification of proliferation changes after treatment with BAY11 at different concentrations (given in μM) in primary VS cells, primary SCs and HEI-193 NF2 VS cell line, all normalized to proliferation in control NT cells (n≥3). C. Representative cell death images are shown for primary VS cultures treated with (a) NT, (b) 1 μM and (c) 5 μM BAY11-7082 (BAY11). TUNEL (green) in nuclei marks dying cells. D. Quantification of cell death rate after treatment with BAY11 at different concentrations (given in μM) in primary VS cells, primary SCs and HEI-193 NF2 VS cell line (n≥3). *p<0.05, **p<0.01, re = compared to. Error bars represent SEM. Nuclei are labeled with Hoechst (blue) in (A, C).