Figure 1. NSPCs in adult mouse subventricular zone express enzymes required for fatty acid oxidation.
Cells expressing Medium Chain Acyl CoA Dehydrogenase (MCAD, A–E) and TriFunctional Protein (TFP, F–J) are observed in the subventricular zone (A, F) and hippocampal dentate gyrus (B, G). A majority of cells expressing these enzymes in SVZ are observed to be co-labelled with SOX2, while none of the cells expressing fatty acid oxidation enzymes in dentate gyrus are labelled with SOX2 (C, H). Higher-magnification images of MCAD+ cells (D–E) and TFP+ cells (I–J) in SVZ (D, I) and DG (E, J) are shown. In SVZ, fewer than 5% of MCAD+ cells co-label with KI67 and fewer than 5% of KI67+ cells co-label with MCAD. Most MCAD+ cells (in red) do not co-label with GFAP+ astrocytes (K–O), S100B+ ependymal cells (P–T), or DCX+ neuroblasts (U–Y). All tissues were stained with the pan-nuclear marker Hoechst (in blue) to demonstrate total cell number. Scale bars represent 20 µm.