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. Author manuscript; available in PMC: 2015 Jun 18.
Published in final edited form as: Nat Commun. 2015 May 26;6:7223. doi: 10.1038/ncomms8223

Figure 1. Transmembrane topology of mPiezo1.

Figure 1

(a) Representative images (3 experimental replicates) of Myc labeling in mPiezo1-Myc transfected HEK293T cells. Myc tags were inserted at position 897 (upper panels) or 753 (lower panels). Immunostaining was performed prior (left panels) or after (right panels) cell permeabilization. Scale bar: 20 μm. (b-c) Schematic of transmembrane topology of mPiezo1 protein showing positions of Myc-tags detected extracellularly (green) and phosphorylated sites detected by mass-spectrometry (blue). Panel (c) also shows positions of Myc-tags detected only after permeabilization (orange).