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. Author manuscript; available in PMC: 2015 May 8.
Published in final edited form as: Methods Mol Biol. 2015;1269:393–412. doi: 10.1007/978-1-4939-2291-8_25

Fig. 1.

Fig. 1

siRNA design rules. (a) An example of target region in an mRNA sequence and the corresponding siRNA duplex with 3′ overhangs. (b) Specific positional rules for siRNA design. The darker cells represent positions on the siRNA antisense (AS) and sense (S) strands. The light gray cells contain specific rules for the corresponding positions. For each set of rules, references are given (Ref). The striped gray background indicates inconsistencies of the rules, due to the different experiments that they come from