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. Author manuscript; available in PMC: 2015 May 8.
Published in final edited form as: Methods Mol Biol. 2015;1269:393–412. doi: 10.1007/978-1-4939-2291-8_25

Fig. 2.

Fig. 2

miRNA sponge constructs. (a) Basic sponge with six miRNA binding sites separated by 4-nt spacers. (b) Perfect miRNA binding site on a sponge. (c) Bulged miRNA binding site on a sponge. (d) Prototype decoy consisting of a short hairpin molecule where the loop exposes a binding site for an miRNA. (e) Tough decoy (TuD) with two exposed miRNA binding sites. (f) Synthetic tough decoy (S-TuD) consisting of two fully 2′-O-methylated RNA strands exposing an miRNA binding site each