Table 1.

Primers and PCR conditions.

Transcript and primers		PCR conditions
Specific first exon forward primers
NESP55 (Accession no. NMJH6592)
Round 1	5′-GAAGAGTCGAAGGAGCCCAAG-3′	95°C × 6 minutes; 95°C × 30 seconds, 57°C × 30 seconds, 72°C
Round 2	5′-GTCACTAATGGAGGACGCCGT-3′	× 1 minute 45 seconds × 35 cycles; 72°C × 10 minutes
XLαs (Accession no. NM_001077490)
Round 1	5′-GGATGCCTCCGCTGGTTTCAGCATCG-3′	95°C × 6 minutes; 95°C × 30 seconds, 57°C × 30 seconds, 72°C
Round 2	5′-CTTTCTCGTGCAAGCCTTC-3′	× 1 minute 45 seconds × 35 cycles; 72°C × 10 minutes
Gas (Accession no. NM_000516)
Round 1	5′-ATGGGCTGCCTCGGGAACAGT-3′	95°C × 3 minutes; 94°C × 30 seconds, 66°C × 2 minutes, × 39 cycles; 68°C × 5 minutes
Common downstream reverse primers		Product size (bp)
Exon 6		NESP55	XLαs	Gαs
Round 1	5′-TTCGTAGCAGGCACGCACTCC-3′	482	616	451
Round 2	5′-GCCTTGGCATGCTCATAGA-3′	329	517	416
Exon 8
Round 1	5′-GTCCACCTGGAACTTGGTCTC-3′	635	769	604
Round 2	5′-CCAGAAGTCAGGACACGGCAG-3′	489	677	576
Exon 13
Round 1	5′-AGAGCAGCTCGTACTGACGAAG-3′	1,172	1,306	1,141
Round 2	5′-GCGCTGAATGATGTCACGGC-3′	1,027	1,215	1,115