Figure A.2.

Heat map displaying the percent prevalence of malformations and mortality resulting from a five-day embryonic zebrafish exposure to a five-fold concentration response series ranging from 0–50 mg/L metal oxide nanoparticles, bulk controls, and, in the case of zinc, a dissolved zinc equivalent (0–99 mg/L) ionic control (n=32 except ZnO NP in water where n = 96). Exposures were conducted in ultrapure water or embryo medium (EM). The color scale above the heat map indicates the percent prevalence of a particular endpoint assessed in the zebrafish at 24 hours post fertilization (hpf) or 120 hpf. Abbreviations for endpoints assessed at 24 hpf: MO24 = mortality, DP = delayed developmental progression, SM = reduced or excessive frequency of zebrafish spontaneous tail coiling, and NC24= notochord malformation. Abbreviations for endpoints assessed at 120 hpf: YSE= yolk sac edema, AXIS= abnormal body axis curvature, EYE= eye malformation, SNOU= snout malformation, JAW= jaw malformation, OTIC= otic vesicle malformation, PE = pericardial edema, BRAIN= brain malformation, SOMI = abnormal somite development, PFIN = malformed pectoral fins, PIG = hypo or hyper pigmentation, CIRC = abnormal circulation or circulatory vasculature, TRUN = shorted body axis, SWIM = abnormal swim bladder development, NC = notochord malformation, MORT = total mortality, and AFTD = total dead and malformed larvae.