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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Endocr Relat Cancer. 2015 Jun 4;22(4):577–591. doi: 10.1530/ERC-15-0192

Figure 7. Rapamycin inhibits glutaminolysis in ovarian cancer cells.

Figure 7

HEY (A), SKOV3 (B), and IGROV-1(C) cells were treated with various concentrations of rapamycin as indicated in their regular media for 24 h. Treatment with rapamycin reduced the expression of GLS and phosphorylation of p-S6. Rapamycin inhibited GDH activity in HEY cells (D) and blocked the cell proliferation induced by glutamine in the ovarian cancer cells after 60 h treatment (E). Western blotting showed rapamycin inhibited the expression of phosphorylation of p-S6 and GLS induced by glutamine in HEY (F), SKOV3 (G) and IGROV-1 (H) cells after 24 hours treatment. *p < 0.05.