Skip to main content
. Author manuscript; available in PMC: 2015 Oct 19.
Published in final edited form as: Cell Discov. 2015 May 5;1:15001. doi: 10.1038/celldisc.2015.1

Figure 7. Actively Translating Ribosomes Co-localize with mRNA in the Nucleus.

Figure 7

(a) HeLa cells were microinjected with the WT, 39T or Kmut β-globin construct. 35 mins later, cells were treated with PMY and emetine for 5 mins at 37°C. Cells were then treated with digitonin for 2 mins followed by FISH and immunofluorescence with the PMY antibody. (b) HeLa cells were microinjected with the WT or 39T β-globin DNA construct. 20 mins later, these cells were or were not treated with anisomycin for 15 mins followed by PMY and emetine treatment for 5 mins at 37°C. Cells were then treated with digitonin for 2 mins followed by FISH and immunofluorescence with the PMY and Ribo P antibodies.