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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Horm Cancer. 2015 Jun 26;6(5-6):214–224. doi: 10.1007/s12672-015-0230-5

Fig. 1.

Fig. 1

Hypomethylation and overexpression of HIST1H2BE in endocrine-resistant cell breast cancer cells. a Relative mRNA expression of histone variants HIST1H3A, HIST1H3C, HIST1H4B, and HIST2BE was measured by RT-qPCR in C4-12, LTED, and MCF-7 cells and analyzed by Student’s t test. ****p<0.0001. b Results of MBD-PD mapped to the University of California Santa Cruz (UCSC) Genome Browser (hg18). Hypermethylated and hypomethylated regions in C4-12 and LTED relative to MCF-7 are represented by upward black and downward gray bars, respectively. Locations of HIST1H2BE and CpG island are indicated on the bottom. c Results of the bisulfite sequencing assay for HIST1H2BE MCF-7, C4-12, and LTED. Each CpG site is represented by a circle, and the percent of methylation at each site is shown by the fraction of black shading. d Immunoblot using antibody against H2B and Tubulin was used as loading control