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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Horm Cancer. 2015 Jun 26;6(5-6):214–224. doi: 10.1007/s12672-015-0230-5

Fig. 3.

Fig. 3

HIST1H2BE knockdown and overexpression affect growth of breast cancer cells. a Relative mRNA expression of HIST1H2BE knockdown clones (sh1, sh2, sh3) compared with non-infected cells (LTED), and cells infected with scrambled control virus (SCR). Expression was analyzed using one-way ANOVA followed by Tukey’s post-test for multiple comparison (***p<0.001). b Growth curves for control cells and cells with HIST1H2BE knockdown. Values represent means±SEM of 2 experiments performed in technical triplicate. Growth was analyzed using non-linear regression and an exponential growth equation (***p<0.001). c Relative mRNA expression of HIST1H2BE overex-pressing clones (H2, H3) compared with non-transfected MCF-7 cells and cells transfected with vector only (−EV1 and −EV2). Measurement was performed as described in (a). d Growth of overexpressing cells (H2) compared with control cells. Cells were kept in 5 % CSS for 3 days before treatment with 10−9 M estradiol. Measurement was performed as described in (b) (**p<0.05)