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. Author manuscript; available in PMC: 2016 Feb 10.
Published in final edited form as: Nat Commun. 2015 Aug 10;6:7912. doi: 10.1038/ncomms8912

Figure 2. Mtb ΔglpX grows on gluconeogenic carbon sources and has detectable FBPase activity.

Figure 2

(a) Growth of WT Mtb (black), ΔglpX (red) and complemented strain ΔglpX/C (gray) in Sauton's minimal media containing no carbon source, 0.4% glucose, 0.1% glycerol or 0.1% butyrate. Data are representative of three independent experiments. (b) FBPase activity of WT Mtb (black), ΔglpX (red) and complemented strain ΔglpX/C (gray) cell lysates in the absence or presence of lithium chloride using 1.5 mM FBP or 12 mM FBP as substrate. Dashed line indicates limit of detection. Data are means ± standard deviation of three biological replicates.