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. Author manuscript; available in PMC: 2009 Mar 24.
Published in final edited form as: J Neurosci. 2008 Sep 24;28(39):9769–9789. doi: 10.1523/JNEUROSCI.2137-08.2008

Figure 12.

Figure 12

Immunoblots demonstrating Cx45 pull-down with the PDZ2 domain of ZO-1. Lysates of Cx45-transfected HeLa cells were incubated with GST fusion proteins containing either the PDZ1, PDZ2 or PDZ3 domain of ZO-1. A, Immunoblots of pull-down proteins probed with monoclonal anti-Cx45 show detection of Cx45 after pull-down with the PDZ2 domain of ZO-1 (lane 3), but not after pull-down with the PDZ1 or PDZ3 domains of ZO-1 (lane 2 and 4). The Cx45 band in lane 3 corresponds to detection of the same band in homogenate of retina (lane 1). B, Blot in A was stripped and re-probed with anti-GST antibody to show presence and equal loading of PDZ domain fusion proteins.