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. Author manuscript; available in PMC: 2009 Mar 24.
Published in final edited form as: J Neurosci. 2008 Sep 24;28(39):9769–9789. doi: 10.1523/JNEUROSCI.2137-08.2008

Figure 8.

Figure 8

Laser scanning confocal double immunofluorescence showing relationships of Cx45 with ZO-1, ZO-2 and ZONAB in the IPL of adult mouse retina, and Cx36, Cx45 and ZO-1 in the IPL of adult wild-type and Cx36 ko mice. Images show the IPL from inner (bottom) to outer (top) edge, and represent z-stacks of five confocal scans in A-D, and single scans in E-I. Co-localization of green and red labeling is seen as yellow in image overlays. A, The same field (A1-A3) showing a high proportion of Cx45-positive puncta labeled for ZO-1. B, The same set of laser scanning confocal double immunofluorescence images as in A, showing minimal Cx45/ZO-1 co-localization in the IPL after horizontal flipping of the image showing labeling for ZO-1. C, Double immunofluorescence overlay showing lack of Cx45 (green) co-localization with ZO-2 (red). D, Double immunofluorescence overlay showing very few Cx45-positive puncta (green) labeled for ZONAB (red). E, Double immunofluorescence overlay showing the persistence of Cx45/ZO-1 co-localization seen as yellow puncta (arrows) in the IPL of Cx36 ko retina. F-I, Confocal scans showing Cx36-puncta (F) and Cx45-puncta (G) in the IPL of wild-type retina, and an absence of labeling for Cx36 (H) and a reduction of labeling for Cx45 (I) in IPL of Cx36 ko retina. Scale bars, 10 μm.