Table 2.
Quantitative immunofluorescence analysis of Cx45 co-localization relationships with Cx36, ZO-1, ZO-2 and ZONAB in the IPL of adult mouse retina
| Proteins examined | Protein (total puncta counted)1 | Percent co-localization2 |
|---|---|---|
| Cx45 positive for Cx363 | Cx45 (766) | 46 ± 7.2 |
| Cx45 positive for Cx364 | Cx45 (2952) | 87 ± 1.3 |
| Cx45 positive for ZO-1 | Cx45 (737) | 71 ± 2.8 |
| Cx45 positive for ZO-2 | Cx45 (432) | 7.0 ± 2.1 |
| Cx45 positive for ZONAB | Cx45 (472) | 4.7 ± 0.4 |
1
Values in parentheses represent the total number of Cx45-positive or Cx36-positive puncta counted in four to ten separate single scan confocal images of the IPL in retinas from two to four mice.
2
Values represent means ± SEM.
3
Data from mice prepared with 40 ml of fixative, with immediate washout.
4
Data from mice prepared with 20 ml of fixative, with immediate washout.