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. Author manuscript; available in PMC: 2009 Mar 24.
Published in final edited form as: J Neurosci. 2008 Sep 24;28(39):9769–9789. doi: 10.1523/JNEUROSCI.2137-08.2008

Table 3.

Numbers of gap junctions labeled for Cx36, Cx45, or Cx36+Cx45 in FRIL single-replicas of mouse and rat retinas that were double-labeled for Cx36 plus Cx45.

Mouse Rat Total
Antibody Shorter Labeling1 Longer Labeling2 Shorter Labeling
Cx36 408 (92%) 137 (88%) 62 (85%) 607 (90%)
Cx36 + Cx45 29 (7%) 19 (12%) 10 (14%) 58 (9%)
Cx45 5 (1%) 0 (0%) 1 (1%) 6 (1%)
Total 442 (100%) 156 (100% 73 (100%) 671 (100%)
1

Shorter labeling was defined as 1– 4.4 h for primary and 12–17 h for secondary antibody labeling; no significant differences were noted between 1–1.2 h and 4.4 h for primary antibodies.

2

Longer labeling was defined as 5.3– 6.8 h for primary and 21.5 h for secondary antibodies.

Numbers in parentheses indicate percentage of labeled gap junctions found in each labeling protocol. These data do not include the 160-plus Cx36 and Cx36+Cx45-containing gap junctions found in the double replicas (see Table 4).

These data do not include the 160-plus Cx36 and Cx36+Cx45-containing gap junctions found in the double-replicas (See Table 4).