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. Author manuscript; available in PMC: 2015 Sep 8.
Published in final edited form as: Eur J Immunol. 2015 Jul 28;45(9):2542–2552. doi: 10.1002/eji.201545591

Figure 2.

Figure 2

F. pedrosoi spores trigger Dectin-1 and Dectin-2 signaling and IL-6 production. (A) BWZ cells and a subline expressing Dectin-1-CD3ζ (Dectin-1), as well as B3Z cells expressing Dectin-2, Mincle, FcRγ chain, Dectin-2 + FcRγ, MCL + FcRγ, Dectin-2 + MCL + FcRγ, Mincle + FcRγ or Mincle + MCL + FcRγ were stimulated with live F. pedrosoi spores. After 18 h, lacZ activity was measured using a colorimetric assay and expressed as OD 560/620 values. Data are expressed as the mean ± SD of duplicate wells. Data are from single experiments representative of three independent experiments. (B) Bone marrow derived dendritic cells (BMDC) were cocultured with live F. pedrosoi spores for 24 h and IL-6 was detected in the supernatants by ELISA. Numbers above histogram bars indicate the n-fold change versus the corresponding wild-type control. Data are from a single experiment representative of two independent experiments.