Figure 3.

T-cell activation and expansion is modestly regulated by Dectin-1, Dectin-2, MCL, and Card9. Wild type, Myd88^−/−, Card9^−/−, FcRγ^−/−, Dectin-1^−/−, Dectin-2^−/−, Mincle^−/−, and Clec4d^+/+ and Clec4d^−/− mice adoptively received 10⁵ CD4⁺ purified, naïve 1807 Tg cells and were infected with 2 × 10⁶ live F. pedrosoi spores or not. At day 7 postinfection, the popliteal lymph nodes were harvested and the (A) number and (B) frequency of activated (CD44⁺) transferred 1807 (Thy1.1⁺) and endogenous CD4⁺ T cells was enumerated by flow cytometry. (A, B) The values over the bars in the histogram indicated the n-fold change in T-cell numbers versus the corresponding wild-type control mice. (C) The dot plots show the sum of concatenated events from five mice per group and the values indicate the mean of endogenous and 1807 CD4⁺ T cells. Data are expressed as mean + SD of five mice per group from a single experiment representative of two independent experiments. The number and frequency of activated (CD44⁺) T cells was not statistically significant between infected knockout mice versus wild-type controls using the Wilcoxon rank test for nonparametric data.