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. Author manuscript; available in PMC: 2015 Sep 8.

Published in final edited form as: Eur J Immunol. 2015 Jul 28;45(9):2542–2552. doi: 10.1002/eji.201545591

F. pedrosoi-induced T-cell differentiation requires Dectin-2, but is suppressed by Mincle. Wild type, Myd88^−/−, Card9^−/−, FcRγ^−/−, Dectin-1^−/−, Dectin-2^−/−, Mincle^−/−, and Clec4d^+/+ and Clec4d^−/− mice adoptively received 10⁵ CD4⁺ purified, naïve 1807 Tg cells and were infected with 2 × 10⁶ live F. pedrosoi spores or not. At day 7 postinfection, the popliteal lymph nodes were harvested and (A, B) the number and (C) frequencies of (A) IL-17- and (B) IFN-γ-producing T cells enumerated by flow cytometry. The values over the bars in the histogram indicated the n-fold change in T-cell numbers versus the corresponding wild-type control mice. The dot plots show the sum of concatenated events from five mice per group and the numbers indicate the mean of endogenous and 1807 CD4⁺ T cells. Data are expressed as mean + SD of five mice per group from a single experiment representative of two independent experiments. *p < 0.05 versus infected wild-type controls using the Wilcoxon rank test for nonparametric data.

F. pedrosoi-induced T-cell differentiation requires Dectin-2, but is suppressed by Mincle. Wild type, Myd88^−/−, Card9^−/−, FcRγ^−/−, Dectin-1^−/−, Dectin-2^−/−, Mincle^−/−, and Clec4d^+/+ and Clec4d^−/− mice adoptively received 10⁵ CD4⁺ purified, naïve 1807 Tg cells and were infected with 2 × 10⁶ live F. pedrosoi spores or not. At day 7 postinfection, the popliteal lymph nodes were harvested and (A, B) the number and (C) frequencies of (A) IL-17- and (B) IFN-γ-producing T cells enumerated by flow cytometry. The values over the bars in the histogram indicated the n-fold change in T-cell numbers versus the corresponding wild-type control mice. The dot plots show the sum of concatenated events from five mice per group and the numbers indicate the mean of endogenous and 1807 CD4⁺ T cells. Data are expressed as mean + SD of five mice per group from a single experiment representative of two independent experiments. *p < 0.05 versus infected wild-type controls using the Wilcoxon rank test for nonparametric data.