Skip to main content
. Author manuscript; available in PMC: 2016 Jan 19.
Published in final edited form as: Molecules. 2015 Jan 19;20(1):1643–1660. doi: 10.3390/molecules20011643

Figure 4.

Figure 4

Effect of nucleotides and inhibitors on the Hsp90β-p50Cdc37 complex as determined by ELISA. (A) Dose-response experiments for the complex formation between immobilized GST-p50Cdc37 and Hsp90β (unphosphorylated or phosphorylated) in the presence of ADP, ATP, AMP-PNP, NVP-AUY922, gedunin, or H2-GMZ, as indicated. (B) Replot of (A) to compare the effect of phosphorylation on the stability of the complex. The Hsp90-(p)p50Cdc37 complex was probed only against ADP and ATP but not against inhibitors. IC50 values are shown in Table 2.