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. Author manuscript; available in PMC: 2015 Jul 29.
Published in final edited form as: J Vis Exp. 2013 Sep 16;(79):e50623. doi: 10.3791/50623

Figure 2.

Figure 2

Schematic of lipid A isolation procedure. Outline depicts chemical lysis of bacterial cell pellet using single phase Bligh-Dyer mixture, centrifugation of lysate to pellet LPS, mild-acid hydrolysis to liberate lipid A from attached polysaccharide, and final purification of lipid A using two phase Bligh-Dyer extraction.