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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Cell Mol Gastroenterol Hepatol. 2015 Sep 1;1(5):503–515. doi: 10.1016/j.jcmgh.2015.06.008

Figure 2. Quantitative real-time PCR detection of SP induced mature miRNA expression.

Figure 2

Mature miRNAs expression quantification was performed after 0.1 μM SP treatment (0.5, 6, 12 and 24 hours) of NCM460-NK-1R cells. Expression levels of miR-221-5p, miR-221-3p, miR-222-5p, and miR-222-3p were assessed by RT-PCR analysis. *p<0.05, data show mean ± standard deviation of triplicate samples per experimental condition.