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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Plant Biotechnol J. 2015 Jan 9;13(7):884–892. doi: 10.1111/pbi.12309

Figure 3.

Figure 3

Protein targeting to protein storage vacuoles (PSV) was evaluated by ultrastructural immunocytochemistry of the rCV-N presence in sections (2 mm) of soybean cotyledons. (a, b) Subcellular accumulation of the rCV-N in the PSV of transgenic T3 seeds from line CV-N10. The accumulation of rCV-N was restricted to the PSVs (white arrows), with no significant detection of the protein associated to the oil bodies (OB) and the cell wall (CW). (c) The rCV-N could not be detected in the PSVs of non-transgenic seeds, as shown in the experimental control.