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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Plant Biotechnol J. 2015 Jan 9;13(7):884–892. doi: 10.1111/pbi.12309

Figure 4.

Figure 4

Analysis of the rCV-N purification process. (a) Coomassie Blue-stained SDS-PAGE (reducing conditions) of fractions from the purification procedure. Lanes marked as 1: pure, active CV-N produced in E. coli (positive control), 2: SeeBlue Plus2 (Invitrogen) molecular weight standard, 3: total soluble protein, 4: supernatant from 67% ethanol precipitation, 5: resuspended pellet from 50% ethanol precipitation, and 6: pure rCV-N isolated in fraction 38 from reverse phase-HPLC. (b) Western blot (reducing conditions) for detection of CV-N in selected fractions from the purification. Lanes marked as 1: total soluble protein, 2: pure rCV-N isolated in fraction 38 from reverse phase-HPLC, 3: pure CV-N produced in E. coli (positive control) and 4: SeeBlue Plus2 (Invitrogen) molecular weight standard.