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. Author manuscript; available in PMC: 2015 Aug 28.
Published in final edited form as: eNeuro. 2015 Feb 18;2(1):e0052. doi: 10.1523/ENEURO.0052-14.2015

Figure 4.

Figure 4

ACh suppresses glutamatergic spontaneous mEPSCs onto Hcrt+ neurons in the presence of atropine. A, Histograms of mEPSC instantaneous frequency show the time course of the effect of ACh on glutamatergic mEPSCs from synapses impinging onto Hcrt+ neurons. Bars above the graph indicate the time point of the ACh (1 mM) puff. The original recordings indicated by “Baseline”, “Ach”, and “Wash” are displayed in B1, B2, and B3, respectively. Inset shows the full trace of an example mEPSC event. C, Cumulative probability plot of the mEPSC amplitude at baseline and upon ACh application from six cells. Inset shows the mean value of the mEPSC amplitude at three time periods: baseline, under the influence of ACh, and washout. **p < 0.01. D, Interevent intervals of mEPSCs at baseline and upon ACh application from six cells (same cells as in C). Inset shows the mean of instantaneous frequency of the mEPSC at three periods of time: baseline, under the influence of ACh, and after washout.