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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Mol Oncol. 2015 Aug 6;10(1):1–23. doi: 10.1016/j.molonc.2015.07.005

Figure 13. Removal of (His)6-tag increases rLOX-PP-Atto565 uptake, while pretreatment with rLOX-PP decreases its uptake.

Figure 13

In A, DU145 cells and PC3 cells were treated with either rLOX-PP-Atto565 with His-tag (gray bars) or without (His)6-tag (white bars), and uptake levels quantified as a function of time by flow cytometry; n = 3, *, p < 0.005. (B) Cells were pre-treated with unlabeled rLOX-PP, followed by rLOX-PP-Atto56, and uptake of rLOX-PP-Atto565 determined; data are means +/− SD, n = 3, *, two-tailed p < 0.05 (Except in MDA-MB-231). (C) A representative confocal image showed that removal of (His)6-tag increased rLOX-PP-Atto565 uptake in PC3 cells at 3 hours (images were collected with EC-Plan Neofluar 40x/1.30 oil DIC M27 objective).